Cultured mouse embryos metabolize benzo(a)pyrene during early gestation: genetic differences detectable by sister chromatid exchange

Journal Article · Sun Jun 01 00:00:00 EDT 1980 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.77.6.3524· OSTI ID:5098085

Galloway, S M ^[1]; Perry, P E; Meneses, J; Nebert, D W; Pedersen, R A

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1. Univ. of California, San Francisco

Mouse embryos explanted at 7 1/2 or 8 1/2 days of gestation were cultured in medium containing benzo(a)pyrene and supplemented with 5-bromodeoxyuridine to allow detection of sister chromatid exchanges. The murine Ah locus regulates the inducible metabolism of polycyclic hydrocarbons such as benzo(a)pyrene. A high frequency of sister chromatid exchanged was induced by benzo(a)pyrene in embryos from three Ah- responsive inbred strains; there was little or no increase in two Ah-nonresponsive inbred strains. Benzo(a)pyrene also induced sister chromatid exchanges in the Ah-responsive recombinant inbred line B6NXAKN-12 but not in the Ah-nonresponsive recombinant inbred line B6NXAKN-3. Sister chromatid exchange in cultured Ah-responsive mouse embryos was thus shown to be a sensitive assay. These data provide direct evidence that genetically responsive mouse embryos possess the subcellular processes necessary for induction of enzymes that metabolize benzo(a)pyrene to its chemically active form(s). Both the Ah regulatory gene product and the structural gene product therefore appear to be functional at an early embryonic age. Furthermore, this metabolic capacity may play an important role in the damage to embryonic cells by polycyclic hydrocarbons.

• OSTI ID: 5098085
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 77:6; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOLOGICAL VARIABILITY
CELL CULTURES
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CYTOCHROMES
EMBRYONIC CELLS
EMBRYOS
GENETIC EFFECTS
GENETIC VARIABILITY
HYDROCARBONS
IN VITRO
MAMMALS
METABOLIC ACTIVATION
MICE
MUTATIONS
ONTOGENESIS
ORGANIC COMPOUNDS
PIGMENTS
RODENTS
SISTER CHROMATID EXCHANGES
TERATOGENESIS
VERTEBRATES