Aryl hydrocarbon hydroxlyase induction by benzo(a)anthracene: regulatory gene localized to the distal portion of mouse chromosome 17. [Mus musculus molossinus, M. m. castaneus]

Journal Article · Sun Jul 01 04:00:00 EDT 1984 · Genetics; (United States)

OSTI ID:6093519

Legraverend, C; Kaerenlampi, S O; Bigelow, S W; Lalley, P A; Kozak, C A; Womack, J E; Nebert, D W

Aryl hydrocarbon (benzo(a)pyrene) hydroxylase inducibility by benzo(a)anthracene was studied in 29 somatic cell hybrid clones, developed by fusing mouse spleen or peritoneal cells from four different inbred strains with hypoxanthine phosphoribosyltransferase-deficient Chinese hamster E36 cells. Karyotype analysis plus 25 markers assigned to 16 autosomes and the X chromosome were examined. In 28 of the 29 clones, the presence or absence of inducibility is associated with the presence or absence, respectively, of mouse chromosome 17. Liver microsomal aryl hydrocarbon hydroxylase induction by 3-methylcholanthrene or benzo(a)anthracene was assessed in appropriate backcrosses with the Mus musculus molossinus, M.m. castaneus, MOR/Cv, Pl/J.SM/J and DBA/2J inbred strains and in 13 NX8 recombinant inbred lines. Twenty-seven biochemical genetic markers representing all but four autosomes were tested for possible linkage with the hydroxylase inducibility, and no linkage was found. The hepatic Ah receptor was quantitated in 26 BXD recombinant inbred lines; the Ah phenotype did not match exactly any of the more than 70 genes with established strain distribution patterns representing 12 autosomes and at least five unlinked markers. It is concluded that a major gene controlling aryl hydrocarbon hydroxylase inducibility by benzo(a)anthracene is located on chromosome 17.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 6093519
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 107:3; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZANTHRACENE
BIOLOGICAL EFFECTS
BIOLOGICAL MARKERS
BIOLOGY
BODY
CELL CULTURES
CLONE CELLS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
GENETIC ENGINEERING
GENETICS
GLANDS
HYDROCARBONS
HYDROXYLASES
LIVER
MAMMALS
MICE
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
POLYCYCLIC AROMATIC HYDROCARBONS
RODENTS
VERTEBRATES