Altered expression of glycosaminoglycans in metastatic 13762NF rat mammary adenocarcinoma cells

Journal Article · Tue Feb 24 04:00:00 EST 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00378a007· OSTI ID:6793142

Steck, P A; Cheong, P H; Nakajima, M; Yung, W K.A.; Moser, R P; Nicolson, G L

A difference in the expression and metabolism of (/sup 35/S)sulfated glycosaminoglycans between rat mammary tumor cells derived from a primary tumor and those from its metastatic lesions has been observed. Cells from the primary tumor possessed about equal quantities of chondroitin sulfate and heparan sulfate on their cell surfaces but released fourfold more chondroitin sulfate than heparan sulfate into their medium. In contrast, cells from distal metastatic lesions expressed approximately 5 times more heparan sulfate than chondroitin sulfate in both medium and cell surface fractions. This was observed to be the result of differential synthesis of the glycosaminoglycans and not of major structural alterations of the individual glycosaminoglycans. The degree of sulfation and size of heparan sulfate were similar for all cells examined. However, chondroitin sulfate, observed to be only chondroitin 4-sulfate, from the metastases-derived cells had a smaller average molecular weight on gel filtration chromatography and showed a decreased quantity of sulfated disaccharides upon degradation with chondroitin ABC lyase compared to the primary tumor derived cells. Major qualitative or quantitative alterations were not observed for hyaluronic acid among the various 13762NF cells. The metabolism of newly synthesized sulfated glycosaminoglycans was also different between cells from primary tumor and metastases. A pulse-chase kinetics study demonstrated that both heparan sulfate and chondroitin sulfate were degraded by the metastases-derived cells, whereas the primary tumor derived cells degraded only heparan sulfate and degraded it at a slower rate. These results suggested that altered glycosaminoglycan expression and metabolism may be associated with the metastatic process in 13762NF rat mammary tumor cells.

• Research Organization: Univ., of Texas M.D. Anderson Hospital and Tumor Institute, Houston
• OSTI ID: 6793142
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:4; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOHYDRATES
CARCINOMAS
CHONDROITIN
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EVEN-ODD NUCLEI
GLUCOPROTEINS
GLUCOSAMINE
HEMATOLOGIC AGENTS
HEPARIN
HEXOSAMINES
HEXOSES
ION EXCHANGE CHROMATOGRAPHY
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
METASTASES
MOLECULAR WEIGHT
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES