Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Purification and partial characterization of glycosaminoglycans and proteoglycans from cultured rabbit smooth muscle cells

Thesis/Dissertation ·
OSTI ID:5073297
Glycosaminoglycans synthesized by cultured rabbit smooth muscle cells were isolated after incorporation of (/sup 3/H)-glucosamine into glycosaminoglycans in the presence or absence of 10% fetal bovine serum. Glycosaminoglycans were quantitated by two-dimensional electrophoresis after proteolytic digestion of the cell layers and media. The results show that the presence of serum has no effect on the chondroitin sulfate, heparan sulfate and dermatan sulfate content of the cell layers. The incorporation of (/sup 3/H)-glucosamine into hyaluronic acid of the cell layers was three times higher in the presence of serum. In the medium , the quantity of hyaluronic was two times higher in the presence of serum while the other glycosaminoglycans remained unchanged. The incorporation of (/sup 3/H)-glucosamine into hyaluronic acid was unaffected by the presence of serum. Specific proteoglycans were isolated from medium after with (/sup 35/S)-sulfate and (/sup 3/H)-serine by isopycnic ultracentrifugation and chromatography on Sepharose CL-4B and DEAE-cellulose. Preparations contained a chondroitin sulfate proteoglycan, a condroitin sulfate-dermatan sulfate proteoglycan and a heparan sulfate proteoglycan. Glycosaminoglycans and proteoglycans synthesized by rabbit aorta smooth muscle cells are similar to those from human aorta.
Research Organization:
Boston Univ., MA (USA)
OSTI ID:
5073297
Country of Publication:
United States
Language:
English

Similar Records

Regulation of sulfated glycosaminoglycan production by prostaglandin E2 in cultured lung fibroblasts
Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Journal of Laboratory and Clinical Medicine; (USA) · OSTI ID:5478922
Chondroitin sulfate proteoglycan synthesis and reutilization of beta-D-xyloside-initiated chondroitin/dermatan sulfate glycosaminoglycans in fetal kidney branching morphogenesis
Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Dev. Biol.; (United States) · OSTI ID:5878917
Stimulation by concanavalin A of cartilage-matrix proteoglycan synthesis in chondrocyte cultures
Journal Article · Fri Jun 15 00:00:00 EDT 1990 · Journal of Biological Chemistry; (USA) · OSTI ID:6718944

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BLOOD SERUM
CARBOHYDRATES
CELL CULTURES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
EVEN-ODD NUCLEI
GLUCOPROTEINS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
ION EXCHANGE CHROMATOGRAPHY
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
MUSCLES
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RABBITS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES