Chondroitin sulfate proteoglycan synthesis and reutilization of beta-D-xyloside-initiated chondroitin/dermatan sulfate glycosaminoglycans in fetal kidney branching morphogenesis

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Dev. Biol.; (United States)

DOI:https://doi.org/10.1016/0012-1606(89)90054-7· OSTI ID:5878917

Klein, D J; Brown, D M; Moran, A; Oegema, Jr, T R; Platt, J L

Branching morphogenesis and chondroitin sulfate proteoglycan synthesis by explanted fetal mouse kidneys were previously shown to be inhibited by p-nitrophenyl beta-D-xylopyranoside (beta-D-xyloside) while glomerular development and heparan sulfate proteoglycan synthesis were unaffected. The metabolic fate of fetal kidney explant proteoglycans was investigated to determine whether or not recovery of proteoglycan synthesis and morphogenesis occur after exposure to beta-D-xyloside. Chondroitin sulfate proteoglycan synthesis resumed within 4 hr of removal of beta-D-xyloside and was enhanced once beta-D-xyloside-initiated chondroitin/dermatan-/sup 35/SO/sub 4/ glycosaminoglycans (GAGs) were released from the tissue. Radioactivity incorporated into beta-D-xyloside-initiated chondroitin/dermatan-/sup 35/SO/sub 4/ GAGs during labeling in the presence of beta-D-xyloside was reutilized in the synthesis of chondroitin-/sup 35/SO/sub 4/ proteoglycan during a 24-hr chase in nonradioactive medium without beta-D-xyloside. Further, highly purified beta-D-xyloside-initiated chondroitin/dermatan-/sup 35/SO/sub 4/ GAGs were taken up by kidneys more avidly than was free (/sup 35/S)sulfate. These /sup 35/S-GAGs were degraded and reutilized in the synthesis of chondroitin-/sup 35/SO/sub 4/ proteoglycan. Ureteric bud branching resumed 48 hr after beta-D-xyloside was removed from the incubation medium. These findings support the idea that both chondroitin sulfate proteoglycan synthesis and proteoglycan processing may be involved in branching morphogenesis.

• Research Organization: Univ. of Minnesota, Minneapolis (USA)
• OSTI ID: 5878917
• Journal Information: Dev. Biol.; (United States), Journal Name: Dev. Biol.; (United States) Vol. 133:2; ISSN DEBIA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CHONDROITIN
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GLYCOPROTEINS
GLYCOSIDES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LIGHT NUCLEI
MAMMALS
METABOLISM
MICE
MUCOPOLYSACCHARIDES
NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES