Sulfated glycosaminoglycan deposition and processing at the basal epithelial surface in branching and beta-D-xyloside-inhibited embryonic salivary glands
Journal Article
·
· Dev. Biol.; (United States)
The authors investigated whether the inhibition of proteoglycan synthesis and salivary branching morphogenesis by beta-D-xyloside was related to the deposition and processing of newly synthesized glycosaminoglycans at the basal epithelial surface that correlates with normal branching activity. Forty eight-hour cultures of control and 0.5 mM beta-xyloside-treated submandibular rudiments were labeled for 2 hr with (/sup 35/S)sulfate and fixed and processed for autoradiography, immediately or after 2, 4, 6, or 8 hr of postlabeling chase in nonradioactive medium. The data demonstrated that deposition of chondroitin sulfate-rich material at the basal epithelial surface was strikingly reduced in beta-xyloside-treated rudiments, while patterns of label loss during postlabeling chase were not altered.
- Research Organization:
- Kansas State Univ., Manhattan
- OSTI ID:
- 5261781
- Journal Information:
- Dev. Biol.; (United States), Journal Name: Dev. Biol.; (United States) Vol. 1; ISSN DEBIA
- Country of Publication:
- United States
- Language:
- English
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Thu Jun 01 00:00:00 EDT 1989
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OSTI ID:5878917
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OSTI ID:6582921
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
EMBRYONIC CELLS
EPITHELIUM
EVEN-ODD NUCLEI
GLANDS
GLYCOSIDES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MICE
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
RADIOISOTOPES
RODENTS
SALIVARY GLANDS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
VERTEBRATES
XENOBIOTICS
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
EMBRYONIC CELLS
EPITHELIUM
EVEN-ODD NUCLEI
GLANDS
GLYCOSIDES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MICE
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
RADIOISOTOPES
RODENTS
SALIVARY GLANDS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
VERTEBRATES
XENOBIOTICS