Partial reversal by beta-D-xyloside of salicylate-induced inhibition of glycosaminoglycan synthesis in articular cartilage
Journal Article
·
· Arthritis Rheum.; (United States)
While net /sup 35/S-glycosaminoglycan synthesis in normal canine articular cartilage was suppressed by 10(-3)M sodium salicylate to about 70% of the control value, addition of xyloside (10(-6)M-10(-3)M) to the salicylate-treated cultures led to a concentration-dependent increase in glycosaminoglycan synthesis, which rose to 120-237% of controls. Similar results were obtained when /sup 3/H-glucosamine was used to measure glycosaminoglycan synthesis, confirming that salicylate suppresses and xyloside stimulates net glycosaminoglycan synthesis, and not merely sulfation. Salicylate (10-3)M) did not affect the activity of xylosyl or galactosyl transferase prepared from canine knee cartilage, and net protein synthesis was unaltered by either salicylate or xyloside. The proportion of newly synthesized proteoglycans existing as aggregates when cartilage was cultured with xyloside was similar to that in controls, although the average hydrodynamic size of disaggregated proteoglycans and of sulfated glycosaminoglycans was diminished.
- Research Organization:
- Rheumatology Division, Indiana University School of Medicine, Indianapolis
- OSTI ID:
- 6582921
- Journal Information:
- Arthritis Rheum.; (United States), Journal Name: Arthritis Rheum.; (United States) Vol. 25:9; ISSN ARHEA
- Country of Publication:
- United States
- Language:
- English
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Journal Article
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Wed Jun 01 00:00:00 EDT 1983
· Arthritis Rheum.; (United States)
·
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· J. Lab. Clin. Med.; (United States)
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Journal Article
·
Fri Mar 31 23:00:00 EST 1989
· Arthritis Rheum.; (United States)
·
OSTI ID:6252426
Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARTILAGE
CELL CULTURES
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DOGS
ENZYME INHIBITORS
ENZYMES
ESTERS
EVEN-ODD NUCLEI
GLUCOPROTEINS
GLUCOSAMINE
GLYCOSYL TRANSFERASES
HEXOSAMINES
HEXOSES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
METABOLISM
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SALICYLIC ACID
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARTILAGE
CELL CULTURES
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DOGS
ENZYME INHIBITORS
ENZYMES
ESTERS
EVEN-ODD NUCLEI
GLUCOPROTEINS
GLUCOSAMINE
GLYCOSYL TRANSFERASES
HEXOSAMINES
HEXOSES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
METABOLISM
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SALICYLIC ACID
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES