Suppression of glycosaminoglycan synthesis by articular cartilage, but not of hyaluronic acid synthesis by synovium, after exposure to radiation

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Arthritis Rheum.; (United States)

DOI:https://doi.org/10.1002/anr.1780320417· OSTI ID:6252426

Hugenberg, S T; Myers, S L; Brandt, K D

We recently found that injection of 2 mCi of yttrium 90 (90Y; approximately 23,000 rads) into normal canine knees stimulated glycosaminoglycan (GAG) synthesis by femoral condylar cartilage. The present investigation was conducted to determine whether radiation affects cartilage metabolism directly. Rates of GAG synthesis and degradation in normal canine articular cartilage were studied following irradiation. Cultured synovium from the same knees was treated similarly, to determine the effects of irradiation on hyaluronic acid synthesis. Twenty-four hours after exposure to 1,000 rads, 10,000 rads, or 50,000 rads, 35S-GAG synthesis by the cartilage was 93%, 69%, and 37%, respectively, of that in control, nonirradiated cartilage. The effect was not rapidly reversible: 120 hours after exposure to 50,000 rads, GAG synthesis remained at only 28% of the control level. Autoradiography showed marked suppression of 35S uptake by chondrocytes after irradiation. Cartilage GAG degradation was also increased following irradiation: 4 hours and 8 hours after exposure to 50,000 rads, the cartilage GAG concentration was only 66% and 54%, respectively, of that at time 0, while corresponding values for control, nonirradiated cartilage were 90% and 87%. In contrast to its effects on cartilage GAG metabolism, radiation at these levels had no effect on synovial hyaluronic acid synthesis.

• Research Organization: Indiana Univ. School of Medicine, Indianapolis (USA)
• OSTI ID: 6252426
• Journal Information: Arthritis Rheum.; (United States), Journal Name: Arthritis Rheum.; (United States) Vol. 32:4; ISSN ARHEA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560162* -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARTILAGE
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DOGS
EVEN-ODD NUCLEI
GLUCOPROTEINS
GLYCOPROTEINS
HOURS LIVING RADIOISOTOPES
HYALURONIC ACID
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
METABOLISM
MUCOPOLYSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIOISOTOPES
SACCHARIDES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TISSUES
VERTEBRATES
YTTRIUM 90
YTTRIUM ISOTOPES