Glycosaminoglycan synthesis by subpopulations of epithelial cells from a mammary adenocarcinoma
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:6816991
Glycosaminoglycan synthesis by two subpopulations of a mouse mammary tumor cell line was compared. The two sublines express distinctly different growth characteristics in vitro and in vivo which indicate differences in growth regulation. Newly made glycosaminoglycans were recovered from the culture media, the cell surfaces, and residual cellular material. The cell population which grows more aggressively in vivo (+SA subline, a subline that grows in soft agarose) incorporated about 8 times more (/sup 14/C)glucosamine per cell into total glycosaminoglycans than did the slower-growing population (-SA subline, which does not grow in soft agarose). Appropriate control experiments indicated that the apparent difference in rates of synthesis was not due to discrepancies in glucosamine uptake. The main residual cellular molecule labeled was heparan sulfate, but the predominant molecule at the cell surface and in the culture fluid was hyaluronic acid. Overall, +SA cells synthesized more hyaluronic acid and -SA cells synthesized more heparan sulfate; in both cell populations, these two molecules accounted for about 90% of total glycosaminoglycans produced.
- Research Organization:
- Department of Zoology, Washington State University, Pullman
- OSTI ID:
- 6816991
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 42:6; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMAL GROWTH
ANIMAL TISSUES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARCINOMAS
CELL CULTURES
COMPARATIVE EVALUATIONS
CULTURE MEDIA
DISEASES
EPITHELIUM
GLANDS
GLUCOSAMINE
GROWTH
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LABORATORY ANIMALS
MAMMARY GLANDS
MONOSACCHARIDES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
SACCHARIDES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
UPTAKE
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMAL GROWTH
ANIMAL TISSUES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARCINOMAS
CELL CULTURES
COMPARATIVE EVALUATIONS
CULTURE MEDIA
DISEASES
EPITHELIUM
GLANDS
GLUCOSAMINE
GROWTH
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LABORATORY ANIMALS
MAMMARY GLANDS
MONOSACCHARIDES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
SACCHARIDES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
UPTAKE