Inhibition of synthesis of heparan sulfate by selenate: Possible dependence on sulfation for chain polymerization

Journal Article · Fri Jan 01 04:00:00 EST 1988 · FASEB Journal; (USA)

OSTI ID:5153516

Dietrich, C P; Nader, H B ^[1]; Buonassisi, V; Colburn, P ^[2]

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1. Paulist School of Medicine, Sao Paulo (Brazil)
2. W. Alton Jones Cell Science Center, Lake Placid, NY (USA)

Selenate, a sulfation inhibitor, blocks the synthesis of heparan sulfate and chondroitin sulfate by cultured endothelial cells. In contrast, selenate does not affect the production of hyaluronic acid, a nonsulfated glycosaminoglycan. No differences in molecular weight, ({sup 3}H)glucosamine/({sup 35}S)sulfuric acid ratios, or disaccharide composition were observed when the heparan sulfate synthesized by selenate-treated cells was compared with that of control cells. The absence of undersulfated chains in preparations from cultures exposed to selenate supports the concept that, in the intact cell, the polymerization of heparan sulfate might be dependent on the sulfation of the saccharide units added to the growing glycosaminoglycan chain.

• OSTI ID: 5153516
• Journal Information: FASEB Journal; (USA), Journal Name: FASEB Journal; (USA) Vol. 2:1; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL TISSUES
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
CHONDROITIN
DAYS LIVING RADIOISOTOPES
DISACCHARIDES
DRUGS
ELEMENTS
ENDOTHELIUM
EVEN-ODD NUCLEI
GLUCOSAMINE
HEMATOLOGIC AGENTS
HEPARIN
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
INHIBITION
INORGANIC ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MOLECULAR WEIGHT
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYMERIZATION
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SELENIUM
SEMIMETALS
SULFUR 35
SULFUR ISOTOPES
SULFURIC ACID
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS