Increased serum erythropoietin activity in rats following intrarenal injection of nickel subsulfide

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Res. Commun. Chem. Pathol. Pharmacol.; (United States)

OSTI ID:6791147

Hopfer, S M; Sunderman, Jr, F W; Fredrickson, T N; Morse, E E

To investigate the pathopysiologic mechanisms of nickel-induced erythocytosis, serum erythropoietin activities were measured in (a) pooled serum from rats at 2 wk after intrarenal injection of ..cap alpha..Ni/sub 3/S/sub 2/ (5 mg/rat), and (b) pooled serum from control rats at 2 wk after intrarenal injection of sterile NaCl vehicle (0.4 ml/rat). A sensitive erythropoietin bioassay was employed, which entailed repetitive administration of test serums to post-hypoxic polycythemic mice in divided doses (12 s.c. injections of 0.5 ml of serum at 6 h intervals for 3 da; total dose = 6 ml of serum/mouse). The erythropoietin detection limit was approx. = 20 I.U./liter of serum. In mice which received pooled serum from ..cap alpha..Ni/sub 3/S/sub 2/-treated rats, erythrocyte /sup 59/Fe-uptake averaged 28% (S.D. +- 5) (vs 3.7 +- 1.1% in control rats; P < 0.001). Based upon a 7-point calibration plot, the erythropoietin activity in pooled serum from ..cap alpha..Ni/sub 3/S/sub 2/-treated rats averaged 130 I.U./liter (S.D. +- 18) (vs 27 +- 6 I.U./liter in control rats; P < 0.001). In vitro addition of Ni(II) to rat serum (100 ..mu..g/liter) had no effect upon serum erythropoietin activity. This study demonstrates that induction of erythrocytosis in rats by intrarenal injection of ..cap alpha..Ni/sub 3/S/sub 2/ is mediated by increased serum erythropoietin activity.

• DOE Contract Number: EY-76-S-02-3140
• OSTI ID: 6791147
• Journal Information: Res. Commun. Chem. Pathol. Pharmacol.; (United States), Journal Name: Res. Commun. Chem. Pathol. Pharmacol.; (United States) Vol. 23:1; ISSN RCOCB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BLOOD COUNT
BLOOD FORMATION
BLOOD SERUM
BODY
BODY FLUIDS
CHALCOGENIDES
DATA
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ERYTHROCYTES
ERYTHROPOIESIS
ERYTHROPOIETIN
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
INFORMATION
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
ISOTOPES
KIDNEYS
MAMMALS
MATERIALS
NICKEL COMPOUNDS
NICKEL SULFIDES
NUCLEI
NUMERICAL DATA
ORGANS
RADIOISOTOPES
RATS
RODENTS
SULFIDES
SULFUR COMPOUNDS
TIME DEPENDENCE
TRANSITION ELEMENT COMPOUNDS
UPTAKE
VERTEBRATES