Dispersal of solid tumors for flow cytometer (FCM) analysis

Conference · Fri Sep 23 04:00:00 EDT 1977

OSTI ID:6790066

Pallavicini, M G; Cohen, A M; Dethlefsen, L A; Gray, J W

Flow cytometer (FCM) analysis of the DNA content of solid tumors necessitates tumor dispersal into single cell suspensions. Single cell suspensions of S102F mammary tumors are prepared by incubating minced tumor tissue at 37/sup 0/C in a sucrose-salt solution containing sodium tetraphenylboron (TPB), a potassium-complexing reagent. After one hour of incubation, the tumor pieces are passed several times through a plastic syringe to dissociate the remaining undispersed fragments. The cells are incubated for an additional 30 minutes and then filtered through 37 ..mu..m nylon mesh. The tumor cells are washed with saline and fixed in formalin for FCM analysis. Approximately 2 to 8 x 10/sup 7/ cells are obtained per 150 ..mu..l tumor volume. Histograms of the distribution of the DNA content of acriflavin-stained tumor cells show a minimal amount of background debris and cell clumping.

• Research Organization: California Univ., Livermore (USA). Lawrence Livermore Lab.
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6790066
• Report Number(s): UCRL-80105; CONF-770324-9
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGY
CELL FLOW SYSTEMS
CYTOLOGY
DNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
QUANTITY RATIO
SAMPLE PREPARATION
TUMOR CELLS