High efficiency flow cytometer

Journal Article · Sat Jan 01 04:00:00 EST 1977 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/25.7.330729· OSTI ID:6438385

Skogen-Hagenson, M J ^[1]; Salzman, G C; Mullaney, P F; Brockman, W H

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1. Los Alamos Scientific Lab., NM

A flow chamber has been developed which collects about 60% of the total cell fluorescence for analysis compared to about 2.5% for conventional flow systems. The chamber, an ellipsoid of revolution, is gold-plated for increased reflectivity. Fluorochrome-stained cells enter the flow cell directly above the primary focus of the ellipsoid at the rate of 1000 cell/sec. A focused argon-ion laser beam enters the flow cell parallel to the semiminor axis and intersects the cell stream at the primary focus. Fluorescent light emanating from this point is reflected toward the secondary focus, where it exits the chamber for analysis. The high efficiency flow cytometer has been used to obtain nucleotide fluorescence distributions from samples of Micrococcus glutamicus bacteria stained with propidium iodide and of spermatozoa stained by the acriflavine-Feulgen procedure.

• OSTI ID: 6438385
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 25:7; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CELL FLOW SYSTEMS
CYTOLOGICAL TECHNIQUES
DESIGN
EFFICIENCY
FLUORESCENCE
GAMETES
GERM CELLS
LASERS
LUMINESCENCE
MICROCOCCUS
MICROORGANISMS
NUCLEOTIDES
ORGANIC COMPOUNDS
SPERMATOZOA