Microfluorometric analysis of cellular DNA following incorporation of bromodeoxyuridine. [CHO cells]
Bromodeoxyuridine (BrdU) incorporation into cellular DNA has a differential effect on the cell-associated fluorescence of several DNA-specific dyes. After cells were treated with BrdU, flow microfluorometry was used to study the relative increase or decrease in fluorescence of stained cells. Bromodeoxyuridine incorporation into CHO cells increased the fluorescence of mithramycin-, olivomycin-, or chromomycin-stained cells, decreased that of propidium iodide-stained cells, and had little, if any, effect on the fluorescence of acriflavine Feulgen-stained cells. Changes in relative fluorescence of cell-associated dyes are due to changes in the amounts of dye bound to cells with BrdU-substituted DNA. Colorimetric and absorbance measurement of DNA content showed that BrdU does not alter the diploid DNA content of CHO cells; however, BrdU induces perturbations in the distribution of cells about the cell cycle which cause an increase in average DNA content.
- Research Organization:
- Los Alamos Scientific Lab., NM
- OSTI ID:
- 7317414
- Journal Information:
- J. Cell. Physiol.; (United States), Vol. 90:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BUDR
BIOCHEMICAL REACTION KINETICS
DNA
QUANTITATIVE CHEMICAL ANALYSIS
FLUORESCENCE SPECTROSCOPY
SAMPLE PREPARATION
ANIMAL CELLS
BIOLOGICAL EFFECTS
CELL CULTURES
MICROANALYSIS
STAINS
UPTAKE
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BROMOURACILS
CHEMICAL ANALYSIS
DRUGS
EMISSION SPECTROSCOPY
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SPECTROSCOPY
URACILS
550200* - Biochemistry