Microfluorometric analysis of cellular DNA following incorporation of bromodeoxyuridine. [CHO cells]

Journal Article · Tue Mar 01 04:00:00 EST 1977 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1040900308· OSTI ID:7317414

Swartzendruber, D E

Bromodeoxyuridine (BrdU) incorporation into cellular DNA has a differential effect on the cell-associated fluorescence of several DNA-specific dyes. After cells were treated with BrdU, flow microfluorometry was used to study the relative increase or decrease in fluorescence of stained cells. Bromodeoxyuridine incorporation into CHO cells increased the fluorescence of mithramycin-, olivomycin-, or chromomycin-stained cells, decreased that of propidium iodide-stained cells, and had little, if any, effect on the fluorescence of acriflavine Feulgen-stained cells. Changes in relative fluorescence of cell-associated dyes are due to changes in the amounts of dye bound to cells with BrdU-substituted DNA. Colorimetric and absorbance measurement of DNA content showed that BrdU does not alter the diploid DNA content of CHO cells; however, BrdU induces perturbations in the distribution of cells about the cell cycle which cause an increase in average DNA content.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 7317414
• Journal Information: J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 90:3; ISSN JCLLA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BROMOURACILS
BUDR
CELL CULTURES
CHEMICAL ANALYSIS
DNA
DRUGS
EMISSION SPECTROSCOPY
FLUORESCENCE SPECTROSCOPY
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
MICROANALYSIS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
QUANTITATIVE CHEMICAL ANALYSIS
REACTION KINETICS
RIBOSIDES
SAMPLE PREPARATION
SPECTROSCOPY
STAINS
UPTAKE
URACILS