Pulse cytometric analysis of the perturbed cellular kinetics of solid tumors: problems and promises

Conference · Mon Nov 03 04:00:00 EST 1975

OSTI ID:7204293

Dethlefsen, L A; Gray, J W; George, Y S; Johnson, S

Pulse cytometric measurements of the cellular DNA content distributions have been made on cells from normal and hydroxyurea-perturbed mammary tumors from two in vivo lines (Slow and S102F) of the C3H mouse. The tumor cells were dissociated mechanically and stained for DNA according to an acriflavine-Feulgen technique. A comparison of autoradiographic data from the tumor sections and single cell suspensions following pulse labeling with /sup 3/H-TdR showed that the dissociation technique is not biased against cells from any of the various phases of the cell cycle; however the mitotic cells are difficult to recognize morphologically. The fluorescence distributions from the DNA stained cells showed a peak corresponding to noncycling diploid cells and at a higher fluorescence, a classical distribution of cycling tumor cells which are near-tetraploid in DNA content. These features are superimposed on a continuum of fluorescence from stained cellular and nuclear fragments. The changes in the DNA distributions produced by perturbing the tumors with hydroxyurea are compared through mathematical modeling to those expected from kinetic data obtained by the more classical methodology of /sup 3/H-TdR pulse labeling and autoradiography. Both the flow microfluorometric and autoradiographic data show that a single injection of hydroxyurea affects a sizable fraction of the S102F tumor cells. The effects on the Slow tumor cells are not as readily apparent. (auth)

• Research Organization: California Univ., Livermore (USA). Lawrence Livermore Lab.
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 7204293
• Report Number(s): UCRL-77458; CONF-750957-4
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AMIDES
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBONIC ACID DERIVATIVES
CELL CYCLE
CELL PROLIFERATION
CYTOLOGICAL TECHNIQUES
DISEASES
DISTRIBUTION
DNA
FLUORESCENCE
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LUMINESCENCE
MAMMALS
MAMMARY GLANDS
MATHEMATICAL MODELS
MICE
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PULSE TECHNIQUES
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RODENTS
THYMIDINE
TRITIUM
TUMOR CELLS
UREA
VERTEBRATES
YEARS LIVING RADIOISOTOPES