Comparison of mercury arc lamp and laser illumination for flow cytometers

Journal Article · Sun Dec 31 23:00:00 EST 1978 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/27.1.374581· OSTI ID:5883998

Peters, D C

Optical differences between a mercury arc lamp and a laser-illuminated flow cytometer are compared. The distributions of spectral intensities of the two light sources are shown in relation to the excitation characteristics of the fluorescent dyes acriflavine, chromomycin A/sub 3/, mithramycin, ethidium bromide, Hoechst 33258, and 4,6-diamidino-2-phenylindole (DAPI). Fluorescence intensities of microspheres and Hoechst 33258-stained mouse sperm are compared in the two cytometers. The optical efficiencies are similar and depend on the match of the excitation characteristics of the stain with the emission spectra of the light source.

• Research Organization: Univ. of California, Livermore
• OSTI ID: 5883998
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 27:1; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CELL FLOW SYSTEMS
ELECTRICAL EQUIPMENT
EMISSION SPECTRA
EQUIPMENT
FLUORESCENCE
GAMETES
GERM CELLS
HISTOLOGICAL TECHNIQUES
LASERS
LIGHTING SYSTEMS
LUMINESCENCE
LUMINOSITY
MAMMALS
MICE
OPTICAL PROPERTIES
PHYSICAL PROPERTIES
RODENTS
SPECTRA
SPERMATOZOA
STAINS
VERTEBRATES