Chromomycin A/sub 3/ as a fluorescent probe for flow cytometry of human gynecologic samples

Journal Article · Fri Dec 31 23:00:00 EST 1976 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/25.7.70448· OSTI ID:6975402

Jensen, R H

Chemical, physical and optical properties of chromomycin A/sub 3/ are examined so as to ascertain appropriate staining and analysis procedures for flow cytometry of human gynecologic samples. Fluorescence excitation and emission spectra of chromomycin A/sub 3/-stained cervical cells are compared with those of chromomycin a/sub 3/-stained deoxyribonucleic acid. Conditions for deoxyribonucleic acid-specific staining of cervical cells are presented, and staining specificity of cervical cells with chromomycin A/sub 3/ is compared to that obtained with ethidium bromide, propidium iodide and Hoechst 33258. Also presented is a brief review of two parameter flow cytometry as a prescreening procedure for detection of cervical neoplasia. Results of flow cytometry and cell sorting are interpreted based on the deoxyribonucliec acid-specificity of chromomycin A/sub 3/ staining.

• Research Organization: Lawrence Livermore Lab., CA
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6975402
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 25:7; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BINDING ENERGY
BODY
CELL FLOW SYSTEMS
COMPARATIVE EVALUATIONS
DIAGNOSIS
DISEASES
DNA
DRUGS
DYES
ENERGY
FEMALE GENITALS
FLUORESCENCE
LUMINESCENCE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATIENTS
SPECIFICITY