Strategies for choosing a deoxyribonucleic acid stain for flow cytometry of metaphase chromosomes
Requirements for flow cytometry of metaphase chromosomes stained with three deoxyribonucleic acid (DNA)-specific fluorescent dyes - Hoechst 33258, Chromomycin A/sub 3/, and ethidium bromide - are reviewed. Fluorescence properties of these three stains when bound to mitotic cells or to chromosomes in suspension are measured and compared with fluorescence properties when bound to DNA in solution. Conditions are given for high resolution flow cytometry of Chinese hamster chromosomes stained with each of the fluorophors, and histograms are presented that exhibit differences in relative peak position and area. Energy transfer fluorescence between two DNA stains is presented as a potentially useful new parameter for flow cytometry of chromosomes and is illustrated by fluorescence energy transfer from Chromomycin A/sub 3/ to ethidium bromide when simultaneously bound to hamster mitotic cells.
- Research Organization:
- Lawrence Livermore Lab., CA
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 7192197
- Journal Information:
- J. Histochem. Cytochem.; (United States), Vol. 25:8
- Country of Publication:
- United States
- Language:
- English
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