Chromomycin A/sub 3/ as a fluorescent probe for flow cytometry of human gynecologic samples

Journal Article · Sat Jan 01 04:00:00 EST 1977 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/25.7.70448· OSTI ID:6803003

Jenson, R H

Chemical, physical and optical properties of chromomycin A/sub 3/ are examined so as to ascertain appropriate staining and analysis procedures for flow cytometry of human gynecologic samples. Fluorescence excitation and emission spectra of chromomycin A/sub 3/-stained cervical cells are compared with those of chromomycin A/sub 3/-stained deoxyribonucleic acid. Conditions for deoxyribonucleic acid-specific staining of cervical cells are presented, and staining specificity of cervical cells with chromomycin A/sub 3/ is compared to that obtained with ethidium bromide, propidium iodide and Hoechst 33258. Also presented is a brief review of two parameter flow cytometry as a prescreening procedure for detection of cervical neoplasia. Results of flow cytometry and cell sorting are interpreted based on the deoxyribonucleic acid-specificity of chromomycin A/sub 3/ staining.

• Research Organization: Univ. of California, Livermore
• OSTI ID: 6803003
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 25:7; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
550600* -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
BIOMEDICAL RADIOGRAPHY
CELL FLOW SYSTEMS
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DNA
FLUOROSCOPY
GYNECOLOGY
MEDICINE
NEOPLASMS
NUCLEAR MEDICINE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIOLOGY
UROGENITAL SYSTEM DISEASES