Dose response, wavelength dependence, and time course of ultraviolet radiation-induced unscheduled DNA synthesis in mouse skin in vivo

Journal Article · Tue May 01 04:00:00 EDT 1984 · Cancer Res.; (United States)

OSTI ID:6782766

Kodama, K; Ishikawa, T; Takayama, S

A new method for autoradiographic measurement of unscheduled DNA synthesis (UDS) in the skin in vivo after treatment with ultraviolet light (UV) was developed. The skin of the back of ICR mice was shaved and exposed to short-wave UV (254 nm) or UVAB (sunlamp, 270 to 440 nm, predominant emission at 312 nm) at various doses. Immediately after irradiation, an isotonic aqueous solution of (methyl-/sup 3/H)thymidine was injected s.c. into a portion of the skin clamped off with ring-shaped forceps. By this method, dose-dependent UDS was clearly demonstrated as silver grains on various types of cells in the skin in response to 254 nm UV or sunlamp UV. However, the energy values at the two wavelengths required to induce the same UDS level differed by 1 order of magnitude. These findings suggested that this system should be useful for quantitative analysis of UV-induced DNA repair in individual cells of the skin in vivo. By this method, the wavelength difference in transmissibility was studied. Autoradiographic results clearly showed that sunlamp UV could reach deeper sites in the skin than did 254 nm UV. A time course study indicated that UDS was almost complete by 48 hr after 254 nm UV but still persisted at 48 hr after sunlamp UV. These results, together with the differences in transmissibility, support higher tumorigenic activity of sunlamp UV than of 254 nm UV to experimental animals.

• Research Organization: Department of Experimental Pathology, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan
• OSTI ID: 6782766
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 44:5; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FREQUENCY DEPENDENCE
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICE
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
RODENTS
SKIN
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES