Unscheduled DNA synthesis in human skin after in vitro ultraviolet-excimer laser ablation

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12470562· OSTI ID:6240816

Green, H A; Margolis, R; Boll, J; Kochevar, I E; Parrish, J A; Oseroff, A R

DNA damage repaired by the excision repair system and measured as unscheduled DNA synthesis (UDS) was assessed in freshly excised human skin after 193 and 248 nm ultraviolet (UV)-excimer laser ablative incisions. Laser irradiation at 248 nm induced DNA damage throughout a zone of cells surrounding the ablated and heat-damaged area. In contrast, with 193 nm irradiation UDS was not detected in cells adjacent to the ablated area, even though DNA strongly absorbs this wavelength. Our results suggest that the lack of UDS after 193 nm irradiation is due to: ''shielding'' of DNA by the cellular interstitium, membrane, and cytoplasm, DNA damage that is not repaired by excision repair, or thermal effects that either temporarily or permanently inhibit the excision repair processes.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6240816
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 89:2; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

560151* -- Radiation Effects on Animals-- Man
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
LASERS
MAMMALS
MAN
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RADIATION EFFECTS
RADIATIONS
SKIN
ULTRAVIOLET RADIATION
VERTEBRATES