DNA damage in cultured human skin fibroblasts exposed to excimer laser radiation

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · Journal of Investigative Dermatology; (United States)

OSTI ID:5505027

Rimoldi, D; Miller, A C; Freeman, S E; Samid, D ^[1]

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1. Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD (USA)

Ultraviolet excimer lasers are being considered for use in a variety of refractive and therapeutic procedures, the long-term biologic consequences of which are unknown. The effect of sublethal doses of 193-nm laser radiation on cellular DNA was examined in cultured human skin fibroblasts. In contrast to 248 nm, treatments with the 193-nm laser radiation below 70 J/m2 did not cause significant pyrimidine dimer formation in the skin cells. This was indicated by the lack of excision repair activities (unscheduled DNA synthesis assay), and further demonstrated by direct analysis of pyrimidine dimers in DNA from irradiated cells. However, a low level of unscheduled DNA synthesis could be detected following irradiation at 193 nm with 70 J/m2. Both the 193-nm and 248-nm radiation were able to induce chromosomal aberrations, as indicated by a micronucleus assay. A dose-dependent increase in micronuclei frequency was observed 48 and 72 h after laser irradiation. These results indicate that exposure of actively replicating human skin fibroblasts to sublethal doses of either 193- or 248-nm laser radiation can result in genotoxicity.

• OSTI ID: 5505027
• Journal Information: Journal of Investigative Dermatology; (United States), Journal Name: Journal of Investigative Dermatology; (United States) Vol. 96:6; ISSN 0022-202X; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DNA REPAIR
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXCIMER LASERS
EXCISION REPAIR
FIBROBLASTS
GAS LASERS
GENETIC EFFECTS
LASER RADIATION
LASERS
MAMMALS
MAN
MUTATIONS
NUCLEIC ACID REPLICATION
ORGANS
PRIMATES
PYRIMIDINE DIMERS
RADIATIONS
RECOVERY
REPAIR
SENSITIVITY
SKIN
SOMATIC CELLS
VERTEBRATES