Cytotoxicity and mutagenicity of excimer laser radiation

Journal Article · Sat Dec 31 23:00:00 EST 1988 · Lasers in Surgery and Medicine; (USA)

DOI:https://doi.org/10.1002/lsm.1900090503· OSTI ID:5023677

Kochevar, I E ^[1]

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1. Massachusetts General Hospital, Boston (USA)

Excimer laser radiation at 193 nm, 248 nm and 308 nm cause DNA photochemistry. The photobiological effects resulting from exposure of cells to 308 nm and 248 nm radiation appear to be the same as those obtained using low irradiance CW sources at similar wavelengths. This indicates that the high irradiances available from the excimer laser cause the same DNA photochemistry as the lower-irradiance CW sources. Excimer laser radiation at 193 nm causes less cytotoxicity than predicted based on the DNA absorption spectrum. This may result from absorption of 193 nm radiation by protein present between the cell surface, and nuclear DNA, or from less efficient DNA photochemistry using 193 nm radiation. In vitro assays indicate that DNA-damaging effects resulting in cytotoxicity decrease in the order 248 nm greater than 308 nm greater than 193 nm.

• OSTI ID: 5023677
• Journal Information: Lasers in Surgery and Medicine; (USA), Journal Name: Lasers in Surgery and Medicine; (USA) Vol. 9:5; ISSN 0196-8092; ISSN LSMED
• Country of Publication: United States
• Language: English

Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXCIMER LASERS
GAS LASERS
GENETIC EFFECTS
IN VITRO
LASER RADIATION
LASERS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS