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Mutagenic potential of a 193-nm excimer laser on fibroblasts in tissue culture

Journal Article · · Ophthalmology (Rochester, Minn.); (United States)
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In response to concerns regarding possible DNA damage by far ultraviolet radiation during excimer laser corneal surgery, the mutagenic potential of an argon fluoride excimer laser (193 nm) on BALB/3T3 mouse fibroblasts grown in tissue culture was investigated. The cumulative incidence of anaplastic transformation after subablative radiant exposures from 3.5 mJ/cm2/pulse to 13.4 mJ/cm2/pulse was 3.6% of all cell cultures. The incidence of anaplastic transformation in nonirradiated controls was 4.2%. Transformation after exposure to x-ray radiation (60.9 rad) was 98.8%. The difference between the incidence of transformation of nonirradiated controls or excimer-treated cultures compared with x-ray radiation-treated cells was significant P less than 0.0001 (chi square test). In this standard cell line, 193-nm laser energy does not appear to have substantial mutagenic potential.

Research Organization:
Univ. of Miami School of Medicine, FL
OSTI ID:
6352510
Journal Information:
Ophthalmology (Rochester, Minn.); (United States), Journal Name: Ophthalmology (Rochester, Minn.); (United States) Vol. 2; ISSN OPHTD
Country of Publication:
United States
Language:
English

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Related Subjects

550600 -- Medicine
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BODY AREAS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
CORNEA
ELECTROMAGNETIC RADIATION
EYES
FACE
FIBROBLASTS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HEAD
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
LASERS
MAMMALS
MEDICINE
MICE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
RODENTS
SENSE ORGANS
SOMATIC CELLS
SURGERY
THYMIDINE
TISSUE CULTURES
VERTEBRATES
X RADIATION