Photoprotective role of epidermal melanin granules against ultraviolet damage and DNA repair in guinea pig skin
We previously developed a quantitative autoradiographic technique with special forceps for measuring unscheduled DNA synthesis (UDS) in mouse skin after treatment with ultraviolet light in vivo. By this method, we investigated the relationship between the protective role of melanin and UV-induced DNA repair in black-and-white guinea pigs. Flat areas containing a sharp border between pigmented and unpigmented skin were selected. The skin of the selected areas was shaved and irradiated with short-wave UV (254 nm) or UV-AB (270 to 440 nm, emission peak at 312 nm) at various doses. Immediately after irradiation, the skin was clamped off with forceps, and an isotonic aqueous solution of (methyl-/sup 3/H)thymidine was injected s.c. into the clamped off portion. UDS was clearly demonstrated as silver grains in this portion of the skin after irradiation with 254 nm UV or UV-AB. Errors due to individual differences were avoided by comparing the intensities of UDS in basal cells from pigmented skin and unpigmented skin of the same animals. Unexpectedly, in groups of animals treated with 254 nm UV or UV-AB, no difference in UDS in pigmented and unpigmented skin was seen at any UV dose. These results suggested that epidermal melanin granules do not significantly protect DNA of basal cells against 254 nm UV or UV-AB irradiation. Results of a study on the effect of the wavelength of irradiation on the UDS response of albino guinea pigs are also reported.
- Research Organization:
- Japanese Foundation for Cancer Research, Tokyo
- OSTI ID:
- 5970442
- Journal Information:
- Cancer Res.; (United States), Vol. 44:11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MELANIN
BIOLOGICAL FUNCTIONS
RADIOSENSITIVITY EFFECTS
SKIN
BIOLOGICAL RADIATION EFFECTS
DNA REPAIR
GUINEA PIGS
RADIATION DOSES
RADIATION PROTECTION
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DOSES
ELECTROMAGNETIC RADIATION
FUNCTIONS
HYDROXY COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PIGMENTS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals