An enzyme-linked immunoabsorbent assay for estimating red cell survival of transfused red cells-validation using CR-51 labeling
Conference
·
· J. Nucl. Med.; (United States)
OSTI ID:6781878
The survival time of transfused red cells antigenically distinct from the recipient's red cells was determined using an indirect enzyme linked antiglobulin test. These results were then compared to those determined by Cr-51 labeling. Three patients with hypoproliferative anemias and one patient (2 studies) with traumatic hemolytic anemia caused by a prosthetic heart valve were studied. Survival times were performed by transfusing a 5cc aliquot of Cr-51 labeled cells along with the remaining unit. One hour post transfusion, a blood sample was drawn and used as the 100% value. Subsequent samples drawn over a 2-3 week period were then compared to the initial sample to determine percent survival for both methods. The ELISA method for measuring red cell survival in antigenically distinct cells is in close agreement with the Cr-51 method. Although CR-51 labeling is the accepted method for red cell survival determination the ELISA method can be used when radioisotopes are unavailable or contraindicated or when the decision to estimate red cell survival is made after transfusion.
- Research Organization:
- The Johns Hopkins Medical Institutions, Baltimore, MD
- OSTI ID:
- 6781878
- Report Number(s):
- CONF-840619-
- Conference Information:
- Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Estimation of transfused red cell survival using an enzyme-linked antiglobulin test
BLOOD VOLUME DETERMINATION AND SURVIVAL TIME OF RED BLOOD CELLS BY MEANS OF RED BLOOD CELLS LABELLED WITH Cr-51
In vivo studies of the long-term /sup 51/Cr red cell survival of serologically incompatible red cell units
Journal Article
·
Sun Sep 01 00:00:00 EDT 1985
· Transfusion (Philadelphia); (United States)
·
OSTI ID:6085402
BLOOD VOLUME DETERMINATION AND SURVIVAL TIME OF RED BLOOD CELLS BY MEANS OF RED BLOOD CELLS LABELLED WITH Cr-51
Journal Article
·
Thu Nov 30 23:00:00 EST 1961
· Primena Radioaktiv. Izotopa i Jonizujucih Zracenja u Med.
·
OSTI ID:4792385
In vivo studies of the long-term /sup 51/Cr red cell survival of serologically incompatible red cell units
Journal Article
·
· Transfusion (Philadelphia); (United States)
·
OSTI ID:5653828
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANEMIAS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL HALF-LIFE
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
MATERIALS
NUCLEI
PATIENTS
RADIOISOTOPES
SURVIVAL TIME
SYMPTOMS
THERAPY
TRACER TECHNIQUES
TRANSFUSIONS
62 RADIOLOGY AND NUCLEAR MEDICINE
ANEMIAS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL HALF-LIFE
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
MATERIALS
NUCLEI
PATIENTS
RADIOISOTOPES
SURVIVAL TIME
SYMPTOMS
THERAPY
TRACER TECHNIQUES
TRANSFUSIONS