In vivo studies of the long-term /sup 51/Cr red cell survival of serologically incompatible red cell units

Journal Article · · Transfusion (Philadelphia); (United States)

OSTI ID:5653828

Baldwin, M L; Ness, P M; Barrasso, C; Kickler, T S; Drew, H; Tsan, M F; Shirey, R S

The long-term survival of serologically incompatible red cell units was measured in five patients with antibodies to high-frequency antigens. Initially, the survival of 1 ml of /sup 51/Cr-labeled incompatible red cells was measured over 1 hour. After demonstrating that the 1-hour survival times were successful (greater than 70%), each patient then received 5 ml of the same /sup 51/Cr-labeled red cells followed by the transfusion of the remainder of the red cell unit. The long-term T 1/2Cr survival for each case was patient 1 (anti-McCa), 15 days; patient 2 (anti-JMH), 12 days; patient 3 (anti-Kna), 31 days; patient 4 (anti-McCa), 12 days; and patient 5 (anti-Hya), 14 days. Each antibody tested in an in vitro homologous macrophage assay showed less than 5 percent phagocytosis. Anti-JMH was the only antibody to react with IgG subclass antisera and was determined to be IgG4. The macrophage assay, IgG subclass testing, and short-term (1 hour, 1 ml) /sup 51/Cr survival studies all indicated that the short-term survival was good. However, only the measurement of long-term survival with transfused units of serologically incompatible red cells was able to determine the actual survival, and clinical significance of the alloantibodies. Determining the actual long-term survival by the method described here can be of importance for patients requiring chronic red cell transfusion.

• Research Organization: Johns Hopkins Medical Institutions, Baltimore, MD
• OSTI ID: 5653828
• Journal Information: Transfusion (Philadelphia); (United States), Journal Name: Transfusion (Philadelphia); (United States) Vol. 25:1; ISSN TRANA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD GROUPS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
EVEN-ODD NUCLEI
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MACROPHAGES
MATERIALS
NUCLEI
PATIENTS
PHAGOCYTES
PHAGOCYTOSIS
RADIOISOTOPES
SOMATIC CELLS
SURVIVAL TIME
THERAPY
TRACER TECHNIQUES
TRANSFUSIONS