Estimation of transfused red cell survival using an enzyme-linked antiglobulin test

Journal Article · Sun Sep 01 04:00:00 EDT 1985 · Transfusion (Philadelphia); (United States)

DOI:https://doi.org/10.1046/j.1537-2995.1985.25586020109.x· OSTI ID:6085402

Kickler, T S; Smith, B; Bell, W; Drew, H; Baldwin, M; Ness, P M

An enzyme-linked antiglobulin test (ELAT) method was developed to estimate survival of transfused red cells. This procedure is based on a principle analogous to that of the Ashby technique were antigenically distinct red cells are transfused and their survival studied. The authors compared the ELAT survival to the V Chromium method (V Cr) in four patients. Three patients with hypoproliferative anemias showed T 1/2 by ELAT of 17.5, 18, and 17 days versus 18.5, 20, and 19 days by the V Cr method. A fourth patient with traumatic cardiac hemolysis had two studies performed. In this case, the ELAT showed a T 1/2 of 10 and 8.1 days while V Cr T 1/2 values were 11 and 10.5 days. The ELAT method for measuring red cell survival yielded data which agreed closely with the results of the V Cr method. Although V Cr is the accepted method for red cell survival, the ELAT method can be used to estimate transfused red cell survival.

• Research Organization: Johns Hopkins School of Medicine, Baltimore, MD
• OSTI ID: 6085402
• Journal Information: Transfusion (Philadelphia); (United States), Journal Name: Transfusion (Philadelphia); (United States) Vol. 5; ISSN TRANA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANEMIAS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COUNT
BLOOD GROUPS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME IMMUNOASSAY
ERYTHROCYTES
EVEN-ODD NUCLEI
HALF-LIFE
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
MATERIALS
NUCLEI
PATIENTS
RADIOASSAY
RADIOISOTOPES
SYMPTOMS