Preparation of iodine-125-labeled insulin for radioimmunoassay: comparison of lactoperoxidase and chloramine-T iodination
Journal Article
·
· J. Nucl. Med.; (United States)
OSTI ID:6759669
The enzymatic radioiodination of porcine insulin by a system consisting of lactoperoxidase, hydrogen peroxide, and Na/sup 125/I was compared with a modified chloramine-T technique. Satisfactory specific activity of the labeled hormone was obtained with the enzymatic iodination, with much greater immunoreactivity and stability than after chloramine-T, besides being quite suitable for the measurement of low plasma insulin levels. There was a positive and highly significant correlation between the insulin concentrations measured with the two tracers, with the regression line defined by the equation: y (chloramine-T) = 8.34 + 0.99 x (lactoperoxidase).
- Research Organization:
- Univ. of Sao Paulo, Brazil
- OSTI ID:
- 6759669
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 19:8; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
CHEMICAL REACTIONS
CHEMISTRY
CHLORAMINES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HALOGENATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
IMMUNOLOGY
INSULIN
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PEROXIDASES
PORPHYRINS
RADIOASSAY
RADIOCHEMISTRY
RADIOIMMUNOASSAY
RADIOISOTOPES
STABILITY
SYNTHESIS
TRACER TECHNIQUES
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
CHEMICAL REACTIONS
CHEMISTRY
CHLORAMINES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HALOGENATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
IMMUNOLOGY
INSULIN
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PEROXIDASES
PORPHYRINS
RADIOASSAY
RADIOCHEMISTRY
RADIOIMMUNOASSAY
RADIOISOTOPES
STABILITY
SYNTHESIS
TRACER TECHNIQUES