Iodination of (Tyr8)-bradykinin-comparison of chloramine-T and lactoperoxidase techniques

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Immunoassay; (United States)

DOI:https://doi.org/10.1080/01971528408062997· OSTI ID:6729050

Redman, L W; Tustanoff, E R

Antigen-antibody kinetics were studied using a hapten which was iodinated by two unique procedures. Using bradykinin, a vasopressor hormone as a model peptide, radioactive iodination (/sup 125/I) of its 8-tyrosyl analogue was carried out both enzymatically and chemically using modified procedures. Two distinct chemical species were obtained which were characterized on a chromatographic, chemical as well as charge basis as a mono-iodinated form of (Tyr8)-bradykinin using the lactoperoxidase procedure and a di-iodinated entity using chloramine-T technique. The addition of a second iodine atom to the antigen lowers its immunoreactivity for its antibody and thus alters the kinetics of this reaction. Further experiments on the stability (temperature, time of storage, and chemical environment) of these iodinated peptides are described.

• Research Organization: Department of Clinical Pathology, Victoria Hospital, London, Ontario, Canada
• OSTI ID: 6729050
• Journal Information: J. Immunoassay; (United States), Journal Name: J. Immunoassay; (United States) Vol. 5:1-2; ISSN JOUID
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BRADYKININ
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
KININS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES