Interference of endogenous lactoperoxidase antibodies in a solid-phase immunosorbent radioassay for antibodies to protein hormones
Journal Article
·
· Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:5948369
Interference of endogenous antibodies to lactoperoxidase (EC 1.11.1.7) has been demonstrated in a solid-phase immunosorbent radioassay for the detection of antibodies to protein hormones. On enzymic iodination with lactoperoxidase, a little of the /sup 125/I is incorporated in the enzyme itself by self-iodination. Because the /sup 125/I-labeled lactoperoxidase was not removed from the tracer by chromatography on Sephadex G-50, G-100, and G-200 columns, endogenous antibodies to the enzyme, which were present in 25 of 28 sera from apparently healthy individuals, were detected in conjunction with the measurement of antibodies to growth hormone (somatotropin), prolactin, and thyroglobulin, thus causing false-positive results. Contaminating radioactive lactoperoxidase can be removed by adsorption chromatography on cellulose or by liquid chromatography, and can be avoided by performing the iodination with immobilized lactoperoxidase or with Chloramine-T.
- Research Organization:
- Univ. of Lund, Sweden
- OSTI ID:
- 5948369
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 30:11; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIBODY FORMATION
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
GLOBULINS
GONADOTROPINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
LTH
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PEROXIDASES
PITUITARY HORMONES
PORPHYRINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
STH
THYROGLOBULIN
TRACER TECHNIQUES
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIBODY FORMATION
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
GLOBULINS
GONADOTROPINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
LTH
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PEROXIDASES
PITUITARY HORMONES
PORPHYRINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
STH
THYROGLOBULIN
TRACER TECHNIQUES