Preparation of 2-/sup 125/I-testosterone and its use as ligand for radioimmunoassay in serum
Journal Article
·
· Anal. Lett.; (United States)
A radioiodination technique is described which consists of heating Testosterone (T) with Na/sup 125/I and hydrogen peroxide in acetic acid in sealed vial. The binding capacity of labelled substances was measured after thin layer chromatography of the reaction mixture. One compound was selected for radioimmunoassay on the basis of its binding capacity for anti-T serum. Standard curves utilizing tritiated and radioiodinated T, under identical conditions, were superimposible. Serum hormone concentrations determined by the two tracers were comparable. Accuracy, precision and sensitivity were satisfactory. Mass spectroscopy and nuclear magnetic resonance of the unlabelled iodinated T indicated that iodine is in C-2.
- Research Organization:
- Univ. of Rome, Italy
- OSTI ID:
- 5451568
- Journal Information:
- Anal. Lett.; (United States), Journal Name: Anal. Lett.; (United States) Vol. 15:B4; ISSN ANALB
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:5372518
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANDROGENS
ANDROSTANES
CHEMICAL REACTIONS
CHEMICAL SHIFT
CHROMATOGRAPHY
DATA
DIAGRAMS
EXPERIMENTAL DATA
HALOGENATION
HORMONES
HYDROXY COMPOUNDS
IMAGES
INFORMATION
INHIBITION
IODINATION
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MEDIUM TEMPERATURE
NUMERICAL DATA
ORGANIC COMPOUNDS
PH VALUE
RADIOASSAY
RADIOIMMUNOASSAY
SEPARATION PROCESSES
SPECTRA
STEROID HORMONES
STEROIDS
TESTOSTERONE
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANDROGENS
ANDROSTANES
CHEMICAL REACTIONS
CHEMICAL SHIFT
CHROMATOGRAPHY
DATA
DIAGRAMS
EXPERIMENTAL DATA
HALOGENATION
HORMONES
HYDROXY COMPOUNDS
IMAGES
INFORMATION
INHIBITION
IODINATION
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MEDIUM TEMPERATURE
NUMERICAL DATA
ORGANIC COMPOUNDS
PH VALUE
RADIOASSAY
RADIOIMMUNOASSAY
SEPARATION PROCESSES
SPECTRA
STEROID HORMONES
STEROIDS
TESTOSTERONE
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES