Direct measurement of testosterone in a pediatric center, with use of a radioimmunoassay kit and unextracted serum

Journal Article · Sat Oct 01 04:00:00 EDT 1983 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6982297

Funderburgh, L J; Zipf, W B; Sotos, J F

A commercial (Radioassay Systems) radioimmunoassay kit for testosterone has been evaluated in which a double-antibody method and an 125I tracer are used. The 50-microL serum sample is assayed directly with no pre-extraction step. The assay system has good sensitivity (0.01 microgram/L), good precision (CV 5.2%), and little cross reactivity between testosterone and other androgens. Results compared well with those by a tritiated radioimmunoassay involving extraction. Values obtained for 97 children compared well with published pediatric normal values. Evidently, this kit provides a rapid and reliable measurement of testosterone in serum specimens and is acceptable for use with pediatric patients.

• OSTI ID: 6982297
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 29:10; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AGE GROUPS
ANDROGENS
ANDROSTANES
ANTIBODIES
BETA DECAY RADIOISOTOPES
BLOOD SERUM
CHILDREN
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVALUATION
HALOGEN COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TESTOSTERONE
TRACER TECHNIQUES
TRITIUM COMPOUNDS