Ultraviolet mutagenesis studies of (psi), a cytoplasmic determinant of Saccharomyces cerevisiae

Journal Article · Tue Jul 01 04:00:00 EDT 1980 · Genetics; (United States)

OSTI ID:6741319

Tuite, M F; Cox, B S

uv mutagenesis was used to probe the molecular nature of (psi), a nonmitochondrial cytoplasmic determinant of Saccharomyces cerevisiae involved in the control of nonsense suppression. The uv-induced mutation from (psi/sup +/) to (psi/sup -/) showed characteristics of forward nuclear gene mutation in terms of frequency, induction kinetics, occurrence of whole and sectored mutant clones and the effect of the stage in the growth cycle on mutation frequency. The involvement of pyrimidine dimers in the premutational lesion giving the (psi/sup -/) mutation was demonstrated by photoreactivation. uv-induced damage to the (psi) genetic determinant was shown to be repaired by nuclear-coded repair enzymes that are responsible for the repair of nuclear DNA damage. uv-induced damage to mitochondrial DNA appeared to be, at least partly, under the control of different repair processes. The evidence obtained suggests that the (psi) determinant is DNA.

• Research Organization: Univ. of Oxford, England
• OSTI ID: 6741319
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 95:3; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560130* -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
CYTOPLASM
DIMERS
DNA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
FUNGI
GENE RECOMBINATION
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
KINETICS
MICROORGANISMS
MITOCHONDRIA
MUTAGENESIS
MUTANTS
MUTATION FREQUENCY
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PHOTOREACTIVATION
PLANTS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SENSITIVITY
SURVIVAL CURVES
TRANSCRIPTION
ULTRAVIOLET RADIATION
YEASTS