UV-induced mutagenesis of phage S13 can occur in the absence of the RecA and UmuC proteins of Escherichia coli

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.19.6614· OSTI ID:6286950

Tessman, I

The UV-induced mutagenesis of phage S13 that accompanies Weigle repair is known to require the products of the recA and umuDC genes, as does the UV-induced mutagenesis of the Escherichia coli chromosome. The author found that UV-induced mutagenesis of phage S13 occurred in the absence of both the RecA and UmuC functions when the irradiated phage was photoreactivated. Furthermore, UV-induced phage mutations were produced in a recA- umuC- cell even without photoreactivation and in the absence of any other known UV repair mechanism, at a frequency 29% of that found after photoreactivation and 7% of that found after Weigle repair, implying that DNA synthesis can proceed past a dimer at an unexpectedly high frequency even when unaided by the UmuC-RecA SOS repair functions. The unaided DNA synthesis appears capable of producing mutations in the vicinity of a pyrimidine dimer; by aiding synthesis past a dimer, a repair mechanism may disclose a mutation without having any active role in producing it.

• Research Organization: Purdue Univ., West Lafayette, IN
• OSTI ID: 6286950
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 19; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
MICROORGANISMS
MUTATIONS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
PARASITES
PROTEINS
PYRIMIDINE DIMERS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VIRUSES