X irradiation in G/sub 2/ phase of two-cell mouse embryos in vitro: cleavage, blastulation, cell kinetics, and fetal development
Two-cell mouse embryos were X-irradiated in vitro at late G/sub 2/ phase with 0.12, 0.24, 0.47, 0.94, and 1.88 Gy and cultured to the hatching stage of blastocysts. Doses of 0.94 and 1.88 Gy impaired hatching of blastocysts and reduced the average cell number per embryo at the end of the in vitro incubation. However, some embryos survived and recovered after irradiation with these doses. They showed cell numbers in the range of control values (about 100 cells). Furthermore, expanded blastocysts microscopically unaffected by irradiation (0.94 and 1.88 Gy) were able to develop into normal, healthy mice after transplantation into foster female mice. Immediately after irradiation, 1.88 Gy led to a G/sub 2/ block of two-cell embryos (about 4 hr). Due to the G/sub 2/ block the embryos proceeded with a delay through the cell cycle which followed irradiation. Fifteen hours after irradiation the cell cycle distribution of the irradiated cells differed from that of the controls.G/sub 1/ cells increased and S-phase cells decreased. In the first/second and third/fourth postradiation cell cycles, hypoploidic cells (DNA content < 2c) were found. It is assumed that these cells which lost DNA by forming micronuclei contributed to cell loss. Cell loss apparently occurred at the morula stage (about 20 cells per embryo) and later in the majority of embryos irradiated with 0.94 and 1.88 Gy.
- Research Organization:
- Institut fur Medizinische Strahlenphysik und Strahelenbiologie, Universitatsklinikum Essen, Federal Republic of Germany
- OSTI ID:
- 6722502
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 91:2; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
EMBRYOS
IN VITRO
IONIZING RADIATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MICE
MITOTIC INDEX
ONTOGENESIS
RADIATION EFFECTS
RADIATIONS
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES
X RADIATION