Does tributyl phosphate influence the radiation risk of a highly proliferating system--the early mouse embryo in vitro
Two-cell mouse embryos were exposed in vitro to tributyl phosphate, x rays, or a combination of both. In-vitro development of the embryos was followed microscopically (cleavage to four- and eight-cell embryos, formation of morulae and blastocysts, and hatching of blastocysts). Effects on proliferation were estimated by counting the number of cells per embryo early (48 h p.c. = 48 hours post conceptionem) and late (144 h p.c.) in the preimplantation period. Cytogenetic damage was studied using micronucleus formation as the end point. Tributyl phosphate did not reveal toxic effects up to a concentration of about 5 microM after an exposure time of 18 h. At a concentration of about 15 microM, 50% of late preimplantation embryos showed effects on morphological development and on cell proliferation, and at about 40 microM, 90% of the embryos were affected. Tributyl phosphate did not induce micronuclei. Small effects by x irradiation were observed between 0.25 Gy and 0.5 Gy, depending on the end point measured in the late preimplantation stage. Fifty percent of the embryos were affected by a dose slightly higher than 1 Gy, and 90% after about 4 Gy. No enhancement in risk was found after combined treatment of the embryos with tributyl phosphate and x rays.
- Research Organization:
- Institut fuer Medizinische Strahlenbiologie, Klinikum Essen, Germany, F.R.
- OSTI ID:
- 5603598
- Journal Information:
- Health Phys.; (United States), Journal Name: Health Phys.; (United States) Vol. 53:6; ISSN HLTPA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CELL NUCLEI
CELL PROLIFERATION
ELECTROMAGNETIC RADIATION
EMBRYOS
ESTERS
HERBICIDES
IN VITRO
IONIZING RADIATIONS
MAMMALS
MICE
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
PESTICIDES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RISK ASSESSMENT
RODENTS
SYNERGISM
THIOPHOSPHORIC ACID ESTERS
TOXICITY
VERTEBRATES
X RADIATION