Differential sensitivity of preimplantation mouse embryos in vitro to x irradiation

Journal Article · Thu Aug 01 00:00:00 EDT 1974 · Biol. Reprod., v. 11, no. 1, pp. 18-21

DOI:https://doi.org/10.1095/biolreprod11.1.18· OSTI ID:4153289

Kirkpatrick, J F

A technique was established for in vitro culture of preimplantation mouse embryos, and their development under these conditions was characterized. The embryos were recovered from superovulated mice, cultured and x-irradiated at various stages of development. Preimplantation embryos were also irradiated and grown in vivo. The investigation included a measurement of differential sensitivity of various stages of development and a comparison of the effects of radiation delivered to embryos in vivo and in vitro. The following LD$sub 50$ values (based on development to the blastocyst) were determined for embryos irradiated in vitro: 390 rads, two-cell stage, 570 rads, four-cell stage; 325 rads, morula stage. The LD$sub 50$ values for embryos irradiated and grown in vitro were: 420 rads, two-cell stage; 640 rads, four-cell stage; 340 rads, morula stage. (auth)

• Research Organization: Cornell Univ., Ithaca, NY
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-003260
• OSTI ID: 4153289
• Journal Information: Biol. Reprod., v. 11, no. 1, pp. 18-21, Journal Name: Biol. Reprod., v. 11, no. 1, pp. 18-21; ISSN BIREB
• Country of Publication: United States
• Language: English

Related Subjects

*EMBRYOS-- RADIOSENSITIVITY
*RADIOSENSITIVITY-- TIME DEPENDENCE
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
BIOLOGICAL RADIATION EFFECTS
COMPARATIVE EVALUATIONS
IN VITRO
IN VIVO
IRRADIATION
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
X RADIATION