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Cell cycle-dependent radiosensitivity in two-cell mouse embryos in culture

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575399· OSTI ID:5300345

The radiosensitivity in embryo systems varies depending on factors such as genetic background, oxygen environment, developmental stage, and age of the embryo in cell cycle. This paper is concerned with the involvement of cell cycle age in radiosensitivity of two-cell mouse embryos. Thus the doses needed for 50% killing of blastocyst formation in vitro (LD/sub 50/) of X rays for the two-cell mouse embryos in culture were measured during their cell cycle. The cell cycle in the two-cell embryos was quite peculiar; the cell cycle time of 18 h was divided into a long DNA post synthesis phase (G/sub 2/) plus mitosis (M) of 14 h and a short DNA synthesis phase (S) of 4 h. Results indicate that the LD/sub 50/ varies roughly from 100 to 600 rad within the cell cycle. Thus a major factor in determining the sensitivity to ionizing radiation of two-cell mouse embryos in vitro and perhaps in vivo is their position in the cell division cycle at the time of irradiation.

Research Organization:
Tokyo Medical and Dental Univ., Japan
OSTI ID:
5300345
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 81:2; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
CELL CULTURES
CELL CYCLE
CELL DIVISION
CRYOGENIC FLUIDS
DATA
DATA FORMS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELEMENTS
EMBRYOS
EXPERIMENTAL DATA
FLUIDS
GENETICS
INFORMATION
IONIZING RADIATIONS
ISOLATED VALUES
MAMMALS
MICE
MITOSIS
NONMETALS
NUCLEIC ACIDS
NUMERICAL DATA
ONTOGENESIS
ORGANIC COMPOUNDS
OXYGEN
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RODENTS
TIME DEPENDENCE
VERTEBRATES
X RADIATION