Mouse embryo attachment to substratum and interaction of trophoblast with cultured cells

Journal Article · Fri Jun 01 04:00:00 EDT 1979 · J. Exp. Zool.; (United States)

DOI:https://doi.org/10.1002/jez.1402080309· OSTI ID:5371907

Glass, R H; Spindle, A I; Pedersen, R A

Hatching, attachment, and trophoblast outgrowth of mouse embryos in vitro were examined as a model for implantation. Mouse embryos attached and grew out on glass cover slips that were partially covered with cultured mouse cells (L cells, liver cells, transformed JLS-V11 cells, and teratocarcinoma cells). Scanning electron microscopy showed that processes of these cells made contact with trophoblast, but there was no evidence of cell lysis or of phagocytosis of the cells by trophoblast. Time-lapse cinematography showed that after contact the cultured mouse cells retracted from the trophoblast, which then spread into the areas vacated by those cells. This suggests a means by which the trophoblast gains entry into the endometrium without destruction of maternal cells. Neuraminidase (100 or 250 units/ml) had no effect on attachment of mouse embryos to glass. However, attachment was inhibited by trypsin at concentrations of 0.25%, 0.025%, and 0.0025%. Treatment of early blastocysts with diazooxo-norleucine, an inhibitor of glycoprotein synthesis, decreased the number of embryos hatching from the zona pellucida; treatment at the late blastocyst stage decreased hatching to a lesser extent. Among the late blastocysts that did hatch, the number forming trophoblast outgrowths was lower than in controls. These results suggest that glycoproteins may be of importance for embryo hatching, attachment, and outgrowth.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5371907
• Journal Information: J. Exp. Zool.; (United States), Journal Name: J. Exp. Zool.; (United States) Vol. 208:3; ISSN JEZOA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CELL CULTURES
CLONE CELLS
EMBRYOS
GLUCOPROTEINS
GROWTH
KINETICS
L CELLS
MAMMALS
MICE
MORPHOLOGY
ORGANIC COMPOUNDS
PHYSIOLOGY
PROTEINS
REACTION KINETICS
REPRODUCTION
RODENTS
SACCHARIDES
TUMOR CELLS
VERTEBRATES