Analysis of compaction, allocation, and outgrowth in the early mouse embryo
Thesis/Dissertation
·
OSTI ID:6070413
Examination of the process of compaction by cinemicrography, and by the use of cytoskeleton-disrupting agents showed it to be a variable, progressive event, dependent on microfilaments both for cell flattering and for polarization of surface microvilli. Examination of the subsequent cleavage division by cinemicrography revealed that there are three major planes of division, and made possible the determination of their relative frequencies during fourth cleavage. Attachment and trophoblast outgrowth on substrates of extracellular matrix molecules was found to involve a group of 140 kD glycoproteins. Addition to the culture medium of a synthetic peptide containing the Arg-Gly-Asp tripeptide cell recognition sequence of fibronectin inhibited trophoblasts outgrowth, but not attachment, on both laminin and fibronectin. Immunoprecipitation of /sup 125/I surface-labeled embryos revealed that the 140 kD glycoproteins are exposed on the surfaces of embryos during outgrowth formation, but are not detectable immediately after hatching. Immunofluorescence experiments showed that the 140 kD glycoproteins and the cytoskeletal proteins vinculin and talin are enriched on the cell processes and ventral surfaces of trophoblasts cells in embryo outgrowths, in patterns similar to those seen in fibroblasts, and consistent with their role in adhesion of the trophoblasts cells to the substratum.
- Research Organization:
- California Univ., San Francisco (USA)
- OSTI ID:
- 6070413
- Country of Publication:
- United States
- Language:
- English
Similar Records
Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor
Dynamic membrane-ctyoskeletal interactions: specific association of integrin and talin arise in vivo after phorbol ester treatment of peripheral blood lymphocytes
Mouse embryo attachment to substratum and interaction of trophoblast with cultured cells
Journal Article
·
Mon Dec 31 23:00:00 EST 1984
· Cell; (United States)
·
OSTI ID:5617239
Dynamic membrane-ctyoskeletal interactions: specific association of integrin and talin arise in vivo after phorbol ester treatment of peripheral blood lymphocytes
Journal Article
·
Thu Dec 31 23:00:00 EST 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6201521
Mouse embryo attachment to substratum and interaction of trophoblast with cultured cells
Journal Article
·
Fri Jun 01 00:00:00 EDT 1979
· J. Exp. Zool.; (United States)
·
OSTI ID:5371907
Related Subjects
550801* -- Morphology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
CELL DIFFERENTIATION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EMBRYOS
GLYCOPROTEINS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICE
NUCLEI
ODD-EVEN NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
CELL DIFFERENTIATION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EMBRYOS
GLYCOPROTEINS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICE
NUCLEI
ODD-EVEN NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
VERTEBRATES