Dynamic membrane-ctyoskeletal interactions: specific association of integrin and talin arise in vivo after phorbol ester treatment of peripheral blood lymphocytes
Members of the family of transmembrane integral membrane proteins called integrins have been implicated in forming attachments to actin microfilaments of the cytoskeleton. These attachments are though to involve one or more intervening peripheral membrane proteins linked to integrin. To detect such possible linkages in vivo, the integrin molecules on the surfaces of intact chicken peripheral blood specific antibodies, and the capped cells were examined by double immunofluorescence to determine whether particular cytoskeletal proteins were co-collected with the integrin. With resting lymphocytes, the capping of integrin did not result in any detectable redistribution of either talin, vinculin, or ..cap alpha..-actinin inside the cells. However, if the capping was carried out upon the addition of phorbol 12-myristate 13-acetate (PMA) to the cells, then talin, but not vinculin or ..cap alpha..-actinin, was found associated with the integrin caps. PMA is known to activate protein kinase C. These results suggest that after, but not before, PMA stimulation of intact cells, talin becomes linked either directly or indirectly with integrin, reflecting the formation of a membrane-cytoskeletal association that is metabolically regulated.
- Research Organization:
- Univ. of California, La Jolla (USA)
- OSTI ID:
- 6201521
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:2
- Country of Publication:
- United States
- Language:
- English
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ANTIBODIES
CROSS-LINKING
CELL MEMBRANES
MOLECULAR STRUCTURE
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PROTEINS
CHICKENS
FLUORESCENCE
IMMUNOLOGY
IN VIVO
LYMPHOCYTES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BIRDS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
ESTERS
FOWL
LEUKOCYTES
LUMINESCENCE
MATERIALS
MEMBRANES
ORGANIC COMPOUNDS
POLYMERIZATION
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology