X-ray-induced dominant lethal mutations in mouse oocytes detected by an in vitro assay
Female mice were X-irradiated with 0.5-4.5 Gy 2 h before mating to unirradiated males of the same strain. The dominant lethal frequencies (DLF) were determined by growing the embryos in vitro from the two-cell stage and determining the relative rates of successful embryogenesis to the blastocyst stage and to the trophectoderm outgrowth with proliferated inner cell mass stage. The DLF increased with increasing dose, the two linear aspects having a breakpoint at about 1.5 Gy. The nature of embryo failure was also dose dependent. At doses less than 2.0 Gy embryos failed predominantly after blastocyst formation, but at higher doses the embryos failed both before and after blastocyst formation. Over the dose range tested, the frequency with which lesions leading to dominant lethality were induced (i.e., -ln(1 - DLF)) increased linearly with increasing dose.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 5650104
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 112:2; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DOMINANT MUTATIONS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EMBRYOS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GERM CELLS
IONIZING RADIATIONS
LETHAL MUTATIONS
MAMMALS
MICE
MUTATION FREQUENCY
MUTATIONS
ONTOGENESIS
OOCYTES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
VERTEBRATES
X RADIATION