Effects of stress, circadian rhythms, and dietary sodium on brain cell-nuclear uptake of aldosterone and corticosterone

Thesis/Dissertation · Mon Dec 31 23:00:00 EST 1984

OSTI ID:6712907

Yongue, B G

The binding of the adrenal steroid hormones aldosterone (ALD) and corticosterone (CORT) in brain cell-nuclei has been implicated as a necessary step in the behavioral and physiological actions of these hormones. In vivo uptake of radioactively labeled ALD and CORT in adrenalectomized (ADX) rats indicates a strong cell-nuclear localization of both of these hormones in limbic brain regions (such as hippocampus, septum and amygdala). Research using sub-cellular fractionation and radioimmunoassay (RIA), has confirmed both the presence of endogenously secreted CORT in cell-nuclei and its limbic localization in the brains of adrenal-intact rats. In this study, environmental and dietary factors were manipulated to induce variation in serum ALD and CORT. A series of experiments employing sub-cellular fractionation and RIA were performed, which reveal that: (1) endogenously secreted ALD and CORT, are concentrated by cell-nuclei of the brain in adrenal-intact rats, (2) the majority of the corticosteroids measured in ethanol extracts of brain cell-nuclei are associated with receptor molecules, and (3) the regional distribution of endogenously secreted ALD differs markedly from the predominantly limbic pattern predicted from in vivo uptake of labeled ALD in ADX rats. Instead, brain cell-nuclear ALD is heavily concentrated in the hypothalamus, which supports the hypothesized relationship between the interaction of ALD and angiotensin in the brain and the behavioral regulation of fluid/electrolyte balance.

• Research Organization: Illinois Univ., Urbana (USA)
• OSTI ID: 6712907
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ALKALI METALS
ANGIOTENSIN
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL LOCALIZATION
BIOLOGICAL STRESS
BODY
BRAIN
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CORTICOSTEROIDS
CORTICOSTERONE
DRUGS
ELEMENTS
GLOBULINS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
METALS
MINERALOCORTICOIDS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIORECEPTOR ASSAY
RATS
RODENTS
SEDIMENTATION
SODIUM
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
UPTAKE
VASOCONSTRICTORS
VERTEBRATES