In vivo radioimmunoassay of aldosterone

Journal Article · Mon Jan 01 04:00:00 EST 1973 · Acta Endocrinol., v. 74, pp. 307-315

DOI:https://doi.org/10.1530/acta.0.0740307· OSTI ID:4330782

Vecsei, P; Gless, K H; Winter, M; Hanka-Postozky, M

Aldosterone antibodies were raised in sheep immunized with a complex of D-aldosterone-21-hemisuccinate and bovine serum albumin. The intravenous injection of these antibodies into rats caused a delay in the disappearance rate of tritiated aldosterone from the blood. The degree of this effect depends on the body weight of the animals, on the amount of the antiserum given, and on the interval between the injection of antibodies and that of labeled aldosterone. After the injection of aldosterone antibodies and the tritiated hormone, the plasma concentration of labeled aldosterone was related to the rate of secretion of the hormone. When aldosterone secretion was low, subsequent to high sodium intake, or abolished after adrenalectomy, the plasma concentration tritiated aldosterone was higher than in the control rats. Stimulation of aldosterone secretion by furosemide or ACTH, or administration of cold aldosterone resulted in a decrease in the plasma concentration of the labeled hormone. It is suggested that the relationship between the amount of free aldosterone in the inner pool and the plasma concentration of tritiated aldosterone is the consequence of a competition of labeled and unlabeled hormone for the binding capacity of the antibody. (auth)

• Research Organization: Univ., Heidelberg
• NSA Number: NSA-29-029965
• OSTI ID: 4330782
• Journal Information: Acta Endocrinol., v. 74, pp. 307-315, Journal Name: Acta Endocrinol., v. 74, pp. 307-315; ISSN ACENA
• Country of Publication: Denmark
• Language: English

Related Subjects

*ALDOSTERONE-- RADIOIMMUNOASSAY
ANTIBODIES
BLOOD PLASMA
CPB
IN VIVO
N48830* --Life Sciences--New Tracer Techniques--Other
RADIONUCLIDE KINETICS
RATS
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS