Intracellular mediators of potassium-induced aldosterone secretion

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90102-W· OSTI ID:6623524

Ganguly, A; Chiou, S; Davis, J S ^[1]

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1. Univ. of South Florida, Tampa (USA)

We have investigated the intracellular messengers of potassium in eliciting aldosterone secretion in calf adrenal glomerulosa cells since there were unresolved issues relating to the role of phosphoinositides, cAMP and protein kinases. We observed no evidence of hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP{sub 2}) in {sup 3}H-inositol labeled alf adrenal cells or increase of cAMP in response to potassium. Addition of calcium channel blocker, nitrendipine after stimulating adrenal glomerulosa cells with potassium, markedly inhibited aldosterone secretion. A calmodulin inhibitor (W-7) produced greater reduction of aldosterone secretion than an inhibitor of protein kinase C (H-7). These results suggest that a rise in cytosolic free calcium concentration through voltage-dependent calcium channel and calmodulin are the critical determinants of aldosterone secretion stimulated by potassium.

• OSTI ID: 6623524
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Journal Issue: 3 Vol. 46:3; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ALKALI METALS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CALMODULIN
CALVES
CATTLE
CORTICOSTEROIDS
DOMESTIC ANIMALS
ELEMENTS
ENDOCRINE GLANDS
ENZYME INHIBITORS
GLANDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
KETONES
KINETICS
MAMMALS
MEMBRANE PROTEINS
METALS
MINERALOCORTICOIDS
ORGANIC COMPOUNDS
ORGANS
PORINS
POTASSIUM
PREGNANES
PROTEINS
REACTION KINETICS
RUMINANTS
SECRETION
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES