Suppressor cells in transplantation tolerance. III. The role of antigen in the maintenance of transplantation tolerance

Journal Article · Sat May 01 00:00:00 EDT 1982 · Transplantation; (United States)

OSTI ID:6705803

Tutschka, P J; Hess, A D; Beschorner, W E; Santos, G W

Suppressor cells, which in an alloantigen-specific manner inhibit proliferation of donor cells to host antigens in a mixed lymphocyte culture and adoptively transfer the suppression of graft-versus-host disease (GVHD), undergo a gradual clonal reduction in long-term, allogeneic, histoincompatible rat radiation chimeras until they can no longer be measured in an in vitro suppressor cell assay. When lymphohematopoietic cells from these chimeras are transferred into lethally irradiated secondary recipients of original donor strain, the suppressor cells, now in a target antigen-free environment, undergo a further clonal reduction. After parking for 120 days, the chimeric cells are specifically tolerant to original host antigens, but cannot adoptively transfer suppression of GVHD. When chimeric cells, parked for 120 days in secondary recipients of original donor strain, are stimulated with original host-type antigen repeatedly during or once at the end of the parking period, the suppressor cell clone is expanded, suppressor cells can be identified in vitro, and suppression of GVHD can adoptively be transferred to tertiary recipients.

• Research Organization: Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, MD
• OSTI ID: 6705803
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 33:5; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CHIMERAS
CLONE CELLS
CONNECTIVE TISSUE CELLS
GRAFT-HOST REACTION
IMMUNOSUPPRESSION
IRRADIATION
LETHAL IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MOSAICISM
RADIATION CHIMERAS
SOMATIC CELLS
TRANSPLANTS