Suppressor cells in transplantation tolerance. II. maturation of suppressor cells in the bone marrow chimera

Journal Article · Thu Oct 01 04:00:00 EDT 1981 · Transplantation; (United States)

OSTI ID:5401578

Tutschka, P J; Ki, P F; Beschorner, W E; Hess, A D; Santos, G W

Histoincompatible bone marrow allografts were established in lethally irradiated rats. At various times after transplantation, the spleen cells were harvested, subjected to mixed lymphocyte cultures, and assayed for suppressor cells in vitro and in vivo by adoptive transfer studies. Alloantigen-nonspecific suppressor cells appeared in the chimera at 40 days after grafting, coinciding with the resolution of graft-versus-host disease (GVHD). At 250 days the nonspecific suppressor cells were replaced by suppressor cells specifically suppressing donor-versus-host alloantigen responses. At 720 days suppressor cells could no longer be identified by in vitro methods but were identified by in vivo adoptive transfer of transplantation tolerance. After injection of host-type antigen into chimeras, the suppressor cells could be again demonstrated by in vitro methods.

• Research Organization: Bone Marrow Transplantation Unit, Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland
• OSTI ID: 5401578
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 32:4; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
BONE MARROW
CELL DIFFERENTIATION
CHIMERAS
GRAFT-HOST REACTION
GRAFTS
HEMATOPOIETIC SYSTEM
IMMUNITY
IN VITRO
IN VIVO
IRRADIATION
LETHAL IRRADIATION
MAMMALS
MOSAICISM
ORGANS
RADIATION CHIMERAS
RADIOINDUCTION
RATS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
TISSUES
TRANSPLANTS
VERTEBRATES