Expression of human. alpha. -fetoprotein in yeast

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90383-3· OSTI ID:6705168

Yamamoto, Ritsu; Sakamoto, Takashi; Nishi, Shinzo; Sakai, Masaharu; Morinaga, Tomonori; Tamaoki, Taiki ^[1]

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1. Hokkaido Univ. School of Medicine (Japan) Univ. of Calgary, Alberta (Canada)

Human {alpha}-fetoprotein (AFP) was expressed in Saccharomyces cerevisiae, with a plasmid containing the cDNA sequence for human AFP fused with the rat AFP signal peptide. The recombinant AFP was purified from the yeast lysate by DEAE-cellulose and immunoaffinity chromatography. The amino acid composition and the molecular weight of the recombinant AFP were similar to those of hepatoma AFP. N-terminal amino acids sequence analysis indicated that the signal peptide had been processed. The recombinant and hepatoma AFP reacted identically in Ouchterlony immunodiffusion and radioimmunoassay tests. These observations indicated that the yeast recombinant protein had the properties of native AFP.

• OSTI ID: 6705168
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 46:23; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BIOASSAY
CELL CONSTITUENTS
CHROMATOGRAPHY
DIAGNOSTIC TECHNIQUES
EUMYCOTA
FUNGI
GENE RECOMBINATION PROTEINS
GENE REGULATION
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MAN
MICROORGANISMS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
PLASMIDS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
YEASTS