The human and mouse homologs of the yeat RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues

Journal Article · Sun Jan 01 04:00:00 EST 1995 · Genomics

DOI:https://doi.org/10.1016/0888-7543(95)80126-7· OSTI ID:241080

Shen, Z; Chen, D J; Denison, K ^[1]

---

1. Los Alamos National Laboratory, NM (United States); and others

The yeast Saccharomyces cerevisiae RAD52 gene is involved in DNA double-strand break repair and mitotic/meiotic recombination. The N-terminal amino acid sequence of yeast S. cerevisiae, Schizosaccharomyces pombe, and Kluyveromyces lactis and chicken is highly conserved. Using the technology of mixed oligonucleotide primed amplification of cDNA (MOPAC), two mouse RAD52 homologous cDNA fragments were amplified and sequenced. Subsequently, we have cloned the cDNA of the human and mouse homologs of yeast RAD52 gene by screening cDNA libraries using the identified mouse cDNA fragments. Sequence analysis of cDNA derived amino acid revealed a highly conserved N-terminus among human, mouse, chicken, and yeast RAD52 genes. The human RAD52 gene was assigned to chromosome 12p12.2-p13 by fluorescence in situ hybridization, R-banding, and DNA analysis of somatic cell hybrids. Unlike chicken RAD52 and mouse RAD51, no significant difference in mouse RAD52 mRNA level was found among mouse heart, brain, spleen, lung, liver, skeletal muscle, kidney, and testis. In addition to an {approximately}1.9-kb RAD52 mRNA band that is present in all of the tested tissues, an extra mRNA species of {approximately}0.85 kb was detectable in mouse testis. 40 refs., 7 figs., 1 tab.

• OSTI ID: 241080
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 25; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
AMINO ACIDS
BANDING TECHNIQUES
CELL DIVISION
DNA HYBRIDIZATION
DNA REPAIR
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE RECOMBINATION
GENE REGULATION
GENES
GENETIC MAPPING
GENETIC RADIATION EFFECTS
HUMAN CHROMOSOME 12
HYBRIDIZATION
IONIZING RADIATIONS
MESSENGER-RNA
MICE
POLYMERASE CHAIN REACTION
SACCHAROMYCES CEREVISIAE
SOMATIC CELLS
STRAND BREAKS